Background:

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

Applications:

ELISA, WB, IHC

Name of antibody:

TRIM27

Immunogen:

Fusion protein of human TRIM27

Full name:

tripartite motif containing 27

Synonyms:

RFP; RNF76

SwissProt:

P14373

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human brain

IHC Recommend dilution:

30-150

WB Predicted band size:

58 kDa

WB Positive control:

Mouse testis tissue and Human testis tissue lysates

WB Recommended dilution:

500-2000