Background:

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:

WB, IHC

Name of antibody:

PAK1(Phospho-Ser204)

Immunogen:

Peptide sequence around phosphorylation site of Serine 204(T-R-S(p)-V-I) derived from Human PAK1.

Full name:

p21 protein (Cdc42/Rac)-activated kinase 1

Synonyms：

PAKalpha

SwissProt:

Q13153

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

61 kDa

WB Positive control:

NIH/3T3 cells lysates treated with UV

WB Recommended dilution:

500-1000